SST3 (IHC-grade), Somatostatin Receptor 3 Antibody

Preview: Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue.

Preview: Immunohistochemical identification of Somatostatin Receptor 3 in human non-functioning pituitary adenoma tissue.

Preview: Immunocytochemical identification of Somatostatin Receptor 3 in transfected HEK293 cells

Preview: Validation of the Somatostatin Receptor 3 in transfected HEK293 cells.

Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue.

Immunohistochemical identification of Somatostatin Receptor 3 in human non-functioning pituitary adenoma tissue.

Immunocytochemical identification of Somatostatin Receptor 3 in transfected HEK293 cells

Validation of the Somatostatin Receptor 3 in transfected HEK293 cells.

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Order number: 7TM0357N-IC
Content: 100 µl
Host: Rabbit

The SST3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The SST3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Somatostatin Receptor 3. In can be used to detect total SST3 receptors in Western blots independent of phosphorylation. The SST3 antibody can also be used to isolate and enrich SST3 receptors from tissue lysates. It also detects SST3 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names SST3, SSTR3, Somatostatin Receptor 3 IUPHAR... more

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Alternative Names	SST3, SSTR3, Somatostatin Receptor 3
IUPHAR Target ID	357
UniProt ID	P32745
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal end of carboxyl-terminal tail of human SST3
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Somatostatin Receptor 3 in human... more

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Figure 1. Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 2. Immunohistochemical identification of Somatostatin Receptor 3 in human non-functioning pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 3. Immunocytochemical identification of Somatostatin Receptor 3 in transfected HEK293 cells. HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were either not exposed or exposed to 1 μM SRIF (somatotropin release inhibiting factor) for 30 min and immunocytochemically stained with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:200. Note, SST3 receptors were confined to the plasma membrane in untreated cells (0 min). SST3 receptors were seen in perinuclear clusters of vesicles after 30 min SRIF exposure.

Figure 4. Validation of the Somatostatin Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 3 receptor (SST3) were lysed and immunoblotted with the anti-SST3 antibody (7TM0357N-IC) at a dilution of 1:1000.

Mundschenk J, Unger N, Schulz S, Höllt V, Schulz S, Steinke R, Lehnert H. Somatostatin... more

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Mundschenk J, Unger N, Schulz S, Höllt V, Schulz S, Steinke R, Lehnert H. Somatostatin receptor subtypes in human pheochromocytoma: subcellular expression pattern and functional relevance for octreotide scintigraphy. J Clin Endocrinol Metab. 2003 Nov;88(11):5150-7. PubMed PMID: 14602742.

Lupp A, Nagel F, Doll C, Röcken C, Evert M, Mawrin C, Saeger W, Schulz S. Reassessment of sst3 somatostatin receptor expression in human normal and neoplastic tissues using the novel rabbit monoclonal antibody UMB-5. Neuroendocrinology. 2012;96(4):301-10. doi: 10.1159/000337659. Epub 2012 May 4. PubMed PMID: 22414742.

Lee M, Lupp A, Mendoza N, Martin N, Beschorner R, Honegger J, Schlegel J, Shively T, Pulz E, Schulz S, Roncaroli F, Pellegata NS. SSTR3 is a putative target for the medical treatment of gonadotroph adenomas of the pituitary. Endocr Relat Cancer. 2015 Feb;22(1):111-9. doi: 10.1530/ERC-14-0472. Epub 2014 Dec 16. PubMed PMID: 25515731.

Lehmann A, Kliewer A, Günther T, Nagel F, Schulz S. Identification of Phosphorylation Sites Regulating sst3 Somatostatin Receptor Trafficking. Mol Endocrinol. 2016 Jun;30(6):645-59. doi: 10.1210/me.2015-1244. Epub 2016 Apr 21. PubMed PMID: 27101376; PubMed Central PMCID: PMC5414643.