pT348-SST3 (phospho-Somatostatin Receptor 3 Antibody)

Preview: Analysis of dose-dependent Somatostatin Receptor 3 phosphorylation.

pT348-SST3 (phospho-Somatostatin Receptor 3 Antibody)

Analysis of dose-dependent Somatostatin Receptor 3 phosphorylation.

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Order number: 7TM0357B
Content: 100 µl
Host: Rabbit

Threonine348 (T348) is a major phosphorylation site of the somatostatin receptor 3 (SST3). The... more

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Threonine348 (T348) is a major phosphorylation site of the somatostatin receptor 3 (SST3). The pT348-SST3 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. T348 phosphorylation is a key regulator of SST3 desensitization, β-arrestin recruitment and internalization.

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Alternative Names SST3, SSTR3, Somatostatin Receptor 3 IUPHAR... more

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Alternative Names	SST3, SSTR3, Somatostatin Receptor 3
IUPHAR Target ID	357
UniProt ID	P32745
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human SST3 around the phosphorylation site of Thr348
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine348 phosphorylation of the Somatostatin Receptor 3. Upper... more

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Figure 1. Agonist-induced Threonine348 phosphorylation of the Somatostatin Receptor 3. Upper panel, HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were either not exposed or exposed to 1 μM SRIF (somatotropin-release inhibiting factor) or 1 μM of artificial somatostatin receptor agonist Octreotide for 30 minutes. Cells were lysed and immunoblotted with the anti-pT348-SST3 antibody (7TM0357B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-SST3 antibody (7TM0357N-WB) at a dilution of 1:1000 to confirm equal loading of the gel. Note, SST3 receptor forms stable dimers.

Figure 2. Analysis of dose-dependent Somatostatin Receptor 3 phosphorylation. Upper panel, HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were either not exposed or exposed to increasing concentrations of SRIF (somatotropin-release inhibiting factor) ranging from 1 nM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT348-SST3 antibody (7TM0357B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-SST3 antibody (7TM0357N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Lehmann A, Kliewer A, Günther T, Nagel F, Schulz S. Identification of Phosphorylation Sites... more

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Lehmann A, Kliewer A, Günther T, Nagel F, Schulz S. Identification of Phosphorylation Sites Regulating sst3 Somatostatin Receptor Trafficking. Mol Endocrinol. 2016 Jun;30(6):645-59. doi: 10.1210/me.2015-1244. Epub 2016 Apr 21. PubMed PMID: 27101376; PubMed Central PMCID: PMC5414643.

Kliewer A, Mann A, Petrich A, Pöll F, Schulz S. A transplantable phosphorylation probe for direct assessment of G protein-coupled receptor activation. PLoS One. 2012;7(6):e39458. doi: 10.1371/journal.pone.0039458. Epub 2012 Jun 26. PubMed PMID: 22745760; PubMed Central PMCID: PMC3383726.

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