ACKR4 (non-phospho), Atypical Chemokine Receptor 4 Antibody

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Preview: Validation of Atypical Chemokine Receptor 4 in transfected HEK293 cells

Preview: Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen

Validation of Atypical Chemokine Receptor 4 in transfected HEK293 cells

Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen

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Order number: 7TM0315N
Content: 100 µl
Host: Rabbit

The ACKR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The ACKR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Atypical Chemokine Receptor 4. It can be used to detect total ACKR4 receptors in Western blots independent of phosphorylation. The ACKR4 antibody can also be used to detect ACKR4 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.

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Alternative Names ACKR4, Atypical Chemokine Receptor 4 IUPHAR Target... more

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Alternative Names	ACKR4, Atypical Chemokine Receptor 4
IUPHAR Target ID	315
Uniprot ID	Q9NPB9
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal end of carboxyl-terminal tail of human ACKR4.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of Atypical Chemokine Receptor 4 in transfected HEK293 cells. Native... more

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Figure 1. Validation of Atypical Chemokine Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 4 (ACKR4) were lysed and immunoblotted with the anti-ACKR4 antibody (7TM0315N-IC) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR4 (Atypical Chemokine Receptor 4) antibody (7TM0315N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ACKR4 receptors were detected at the plasma membrane of nearly all cells in human spleen.

Figure 3. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR4 (Atypical Chemokine Receptor 4) antibody (7TM0315N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ACKR4 receptors were detected at the plasma membrane of nearly all cells in human spleen.

Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical... more

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Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical determination of five somatostatin receptors in meningioma reveals frequent overexpression of somatostatin receptor subtype sst2A. Clin Cancer Res. 2000 May;6(5):1865-74. PubMed PMID: 10815909.

Asnacios A, Courbon F, Rochaix P, Bauvin E, Cances-Lauwers V, Susini C, Schulz S, Boneu A, Guimbaud R, Buscail L. Indium-111-pentetreotide scintigraphy and somatostatin receptor subtype 2 expression: new prognostic factors for malignant well-differentiated endocrine tumors. J Clin Oncol. 2008 Feb 20;26(6):963-70. doi: 10.1200/JCO.2007.12.7431. PubMed PMID: 18281671.

Fischer T, Doll C, Jacobs S, Kolodziej A, Stumm R, Schulz S. Reassessment of sst2 somatostatin receptor expression in human normal and neoplastic tissues using the novel rabbit monoclonal antibody UMB-1. J Clin Endocrinol Metab. 2008 Nov;93(11):4519-24. doi: 10.1210/jc.2008-1063. Epub 2008 Aug 12. PubMed PMID: 18697876.

Lesche S, Lehmann D, Nagel F, Schmid HA, Schulz S. Differential effects of octreotide and pasireotide on somatostatin receptor internalization and trafficking in vitro. J Clin Endocrinol Metab. 2009 Feb;94(2):654-61. doi: 10.1210/jc.2008-1919. Epub 2008 Nov 11. PubMed PMID: 19001514.

Shi TJ, Xiang Q, Zhang MD, Barde S, Kai-Larsen Y, Fried K, Josephson A, Glück L, Deyev SM, Zvyagin AV, Schulz S, Hökfelt T. Somatostatin and its 2A receptor in dorsal root ganglia and dorsal horn of mouse and human: expression, trafficking and possible role in pain. Mol Pain. 2014 Feb 13;10:12. doi: 10.1186/1744-8069-10-12. PubMed PMID: 24521084; PubMed Central PMCID: PMC3943448.

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