pS350/pT352-ACKR3 (phospho-Atypical Chemokine Receptor 3 Antibody)

Preview: Analysis of dose-dependent Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 phosphorylation.

pS350/pT352-ACKR3 (phospho-Atypical Chemokine Receptor 3 Antibody)

Analysis of dose-dependent Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 phosphorylation.

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Order number: 7TM0080A
Content: 100 µl
Host: Rabbit

Serine350/Threonine352 (S350/T352) is major phosphorylation site of the Atypical Chemokine... more

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Serine350/Threonine352 (S350/T352) is major phosphorylation site of the Atypical Chemokine Receptor 3 (ACKR3, previously called CXCR7). The pS350/pT352-ACKR3 antibody detects phosphorylation in response to agonists and after PKC activation. S350/T352 phosphorylation is required for efficient ligand sequestration by ACKR3.

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Alternative Names ACKR3, CXCR7, Atypical Chemokine Receptor 3, CXC... more

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Alternative Names	ACKR3, CXCR7, Atypical Chemokine Receptor 3, CXC Chemokine Receptor 7
IUPHAR Target ID	80
UniProt ID	P25106 (human) P56485 (mouse)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human ACKR3 around the phosphorylation site of Ser350/Thr352
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine350/Threonine352 phosphorylation of the Atypical Chemokine... more

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Figure 1. Agonist-induced Serine350/Threonine352 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7. Upper panel, HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3/CXCR7) were either not exposed or exposed to 100 nM CXCL12 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS350/pT352-ACKR3/CXCR7 antibody (7TM0080A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-ACKR3/CXCR7 antibody (7TM0080N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 phosphorylation. Upper panel, HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3/CXCR7) were either not exposed or exposed to increasing concentrations of CXCL12 ranging from 5 to 250 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS350/pT352-ACKR3/CXCR7 antibody (7TM0080A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-ACKR3/CXCR7 antibody (7TM0080N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM,... more

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Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Sánchez-Alcañiz JA, Haege S, Mueller W, Pla R, Mackay F, Schulz S, López-Bendito G, Stumm R, Marín O. Cxcr7 controls neuronal migration by regulating chemokine responsiveness. Neuron. 2011 Jan 13;69(1):77-90. doi: 10.1016/j.neuron.2010.12.006. PubMed PMID: 21220100.

Hoffmann F, Müller W, Schütz D, Penfold ME, Wong YH, Schulz S, Stumm R. Rapid uptake and degradation of CXCL12 depend on CXCR7 carboxyl-terminal serine/threonine residues. J Biol Chem. 2012 Aug 17;287(34):28362-77. doi: 10.1074/jbc.M111.335679. Epub 2012 Jun 26. PubMed PMID: 22736769; PubMed Central PMCID: PMC3436560.