ACKR4 (IHC-grade), Atypical Chemokine Receptor 4 Antibody

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Preview: Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen.

Preview: ACKR4 (IHC-grade), Atypical Chemokine Receptor 4 Antibody

Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen.

ACKR4 (IHC-grade), Atypical Chemokine Receptor 4 Antibody

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Order number: 7TM0315N-IC
Content: 100 µl
Host: Rabbit

The ACKR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The ACKR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Atypical Chemokine Receptor 4. It can be used to detect total ACKR4 receptors in Western blots independent of phosphorylation. The ACKR4 antibody can also be used to detect ACKR4 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.

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Alternative Names ACKR4, Atypical Chemokine Receptor 4 IUPHAR Target... more

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Alternative Names	ACKR4, Atypical Chemokine Receptor 4
IUPHAR Target ID	315
UniProt ID	Q9NPB9
Immunohistochemistry (IHC)	1:100
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal end of carboxyl-terminal tail of human ACKR4
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen.... more

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Figure 1. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR4 (Atypical Chemokine Receptor 4) antibody (7TM0315N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ACKR4 receptors were detected at the plasma membrane of nearly all cells in human spleen.

Figure 2. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR4 (Atypical Chemokine Receptor 4) antibody (7TM0315N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ACKR4 receptors were detected at the plasma membrane of nearly all cells in human spleen.

Figure 3. Validation of Atypical Chemokine Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 4 (ACKR4) were lysed and immunoblotted with the anti-ACKR4 antibody (7TM0315N-IC) at a dilution of 1:1000.

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