GPR139 (non-phospho), G protein-coupled Receptor 139 Antibody

Figure 1. Validation of the GPR139 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR139 Receptor (GPR139) were lysed and immunoblotted with the phosphorylation-independent anti-GPR139 antibody (7TM0130N) at a dilution of 1:1000.

 Figure 2. Immunohistochemical varification of GPR139 Receptor antibody in kidney. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR139 (non-phospho-GPR139 Receptor) antibody (7TM0130N). Sections were then incubated with anti-GPR139 (non-phospho-GPR139 Receptor) antibody (7TM0130N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR139 receptors were uniformly detected at the plasma membrane of nearly all cells.