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- Order number: 7TM0312A
- Content: 100 µl
- Host: Rabbit
Threonine338 (T338) is major phosphorylation site of the Hydroxycarboxylic Acid Receptor 2 (HCA2). The pT338-HCA2 antibody detects phosphorylation in response to agonists. T338 phosphorylation is likely to be involved in efficient ligand sequestration.
| Alternative Names | PUMAG, Nicotinic acid receptor, G-protein coupled receptor 109A, Niacin receptor 1 |
| IUPHAR Target ID | 312 |
| UniProt ID | Q8TDS4 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from human HCA2 around the phosphorylation site of Thr338. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine338 phosphorylation of the Hydroxycarboxylic Acid Receptor 2. Upper panel, HEK293 cells stably expressing the Hydroxycarboxylic acid receptor 2 (HCA2) were either not exposed or exposed to 100 nM Nicotinic Acid for 30 minutes. Cells were lysed and immunoblotted with the anti-pT338-HCA2 antibody (7TM0312A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-HCA2 antibody to confirm equal loading of the gel.
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