pS346/pS347-CXCR4 (phospho-CXC Chemokine Receptor 4 Antibody)

Preview: Analysis of dose-dependent Serine346/Serine347 CXC Chemokine Receptor 4 phosphorylation.

pS346/pS347-CXCR4 (phospho-CXC Chemokine Receptor 4 Antibody)

Analysis of dose-dependent Serine346/Serine347 CXC Chemokine Receptor 4 phosphorylation.

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Order number: 7TM0071B
Content: 100 µl
Host: Rabbit

Serine346/serine347 (S346/S347) is a major phosphorylation site of the CXCR4 receptor. The... more

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Serine346/serine347 (S346/S347) is a major phosphorylation site of the CXCR4 receptor. The pS346/pS347-CXCR4 antibody detects phosphorylation in response to agonists and after PKC activation. S346/S347 phosphorylation is a key regulator of CXCR4 desensitization, β-arrestin recruitment and internalization.

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Alternative Names CXCR4, CXC Chemokine Receptor 4 IUPHAR Target ID... more

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Alternative Names	CXCR4, CXC Chemokine Receptor 4
IUPHAR Target ID	71
UniProt ID	P61073 (human) P70658 (mouse)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser346/Ser347
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4.... more

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Figure 1. Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4. Upper panel, HEK293 cells stably expressing the HA-tagged CXC Chemokine receptor 4 were either not exposed or exposed to 100 nM CXCL12 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346/pS347-CXCR4 antibody (7TM0071B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Serine346/Serine347 CXC Chemokine Receptor 4 phosphorylation. Upper panel, HEK293 cells stably expressing the HA-tagged CXC Chemokine receptor 4 were either not exposed or exposed to increasing concentrations of CXCL12 ranging from 1 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346/pS347-CXCR4 antibody (7TM0071B) at a dilution of 1:1000. Middle panel, blot was stripped and reprobed with the phosphorylation-sensitive anti-CXCR4 antibody (7TM0071N-WB) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000 to confirm equal loading of the gel.