D5 (non-phospho), Dopamine Receptor 5 Antibody

Figure 1. Validation of the Dopamine Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the dopamine receptor 5 (D5) were lysed and immunoblotted with the phosphorylation-independent anti-D5 antibody (7TM0218N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D5 (Dopamine Receptor 5) antibody (7TM0218N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of Dopamine Receptor 5 in human cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D5 (Dopamine Receptor 5) antibody (7TM0218N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

 Figure 4. Immunohistochemical varification of Dopamine Receptor 5 antibody in human cortex. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-D5 (non-phospho-Dopamine Receptor 5) antibody (7TM0218N). Sections were then incubated with anti-D5 (non-phospho-Dopamine Receptor 5) antibody (7TM0218N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation D5 receptors were uniformly detected at the plasma membrane of nearly all cells.

 Figure 5. Immunohistochemical varification of Dopamine Receptor 5 antibody in rat cortex. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-D5 (non-phospho-Dopamine Receptor 5) antibody (7TM0218N). Sections were then incubated with anti-D5 (non-phospho-Dopamine Receptor 5) antibody (7TM0218N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation D5 receptors were uniformly detected at the plasma membrane of nearly all cells.