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- Order number: 7TM0120N
- Content: 100 µl
- Host: Rabbit
The GPR84 antibody is directed against the distal end of the carboxyl-terminal tail of human GRP84. It can be used to detect total GPR84 receptors in Western blots independent of phosphorylation. The GPR84 antibody can also be used to isolate and enrich GPR84 receptors from cell and tissue lysates. It also detects GPR84 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | GPR84, G Protein-coupled Receptor 84 |
| IUPHAR Target ID | 120 |
| Uniprot ID | Q9NQS5 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents distal end of carboxyl-terminal tail of human GPR84. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the G protein-coupled Receptor 84 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 84 (GPR84) were lysed and immunoblotted with the anti-GPR84 antibody (7TM0120N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of G Protein-coupled Receptor 84 in lymph node. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR84 (G Protein-coupled Receptor 84) antibody (7TM0120N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR84 receptors were detected at the plasma membrane of cells in lymph node.
Figure 3. Immunohistochemical identification of G Protein-coupled Receptor 84 in cerebral cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR84 (G Protein-coupled Receptor 84) antibody (7TM0120N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR84 receptors were detected at the plasma membrane of cells in cerebral cortex.
Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G. Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands. J Biol Chem. 2022 May;298(5):101932. doi: 10.1016/j.jbc.2022.101932. Epub 2022 Apr 12. PMID: 35427647; PMCID: PMC9118924.
