pT263/pT264-GPR84 (phospho-GPR84 Antibody)

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Agonist-induced Threonine263/Threonine264 phosphorylation of GPR84

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Order number: 7TM0120B
Content: 100 µl
Host: Rabbit

Threonine263/Threonine264 (T263/T264) is major phosphorylation site of human GPR84 receptor. The... more

Threonine263/Threonine264 (T263/T264) is major phosphorylation site of human GPR84 receptor. The pT263/pT264-GPR84 antibody detects phosphorylation in response to agonists. T263/T264 phosphorylation is likely to be involved in efficient ligand sequestration by GPR84.

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Alternative Names GPR84, G Protein-coupled Receptor 84 IUPHAR Target... more

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Alternative Names	GPR84, G Protein-coupled Receptor 84
IUPHAR Target ID	120
UniProt ID	Q9NQS5
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR84 around the phosphorylation site of Thr263/Thr264.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine263/Threonine264 phosphorylation of GPR84. Upper panel ,... more

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Figure 1. Agonist-induced Threonine263/Threonine264 phosphorylation of GPR84. Upper panel, parental or Flp-In TREx 293 harbouring human GPR84-eYFP induced to express the receptor construct (+ dox) were treated with vehicle or 2-HTP for 5 min. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the pThr263/pThr264-antibody (7TM0120B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GFP84 antibody (7TM0120N) at a dilution of 1:1000 to confirm equal loading of the gel.

Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova... more

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Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G. Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands. J Biol Chem. 2022 May;298(5):101932. doi: 10.1016/j.jbc.2022.101932. Epub 2022 Apr 12. PMID: 35427647; PMCID: PMC9118924.

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