DP1 (non-phospho), DP1 Prostanoid Receptor Antibody

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Preview: Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas

Preview: Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma

Preview: Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney

Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells

Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas

Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma

Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney

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Order number: 7TM0338N
Content: 100 µl
Host: Rabbit

The non-phospho-DP1 Receptor Antibody is directed against the distal end of the... more

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The non-phospho-DP1 Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human DP1. It can be used to detect total DP1 receptors in Western blots independent of phosphorylation. The non-phospho-DP1 antibody can also be used to isolate and enrich DP1 receptors from cell and tissue lysates. It also detects DP1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names DP1, prostanoid DP receptor-like, PTGDR1, prostaglandin... more

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Alternative Names	DP1, prostanoid DP receptor-like, PTGDR1, prostaglandin D2 receptor (DP)
IUPHAR Target ID	388
UniProt ID	Q13258
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human DP1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells . Native... more

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Figure 1. Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the DP1 prostanoid receptor 1 (DP1) were lysed and immunoblotted according to 7TM Western Blot Protocol with the phosphorylation-independent anti-DP1 antibody (7TM0388N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 4. Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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