DP1 (non-phospho), DP1 Prostanoid Receptor Antibody

Figure 1. Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the DP1 prostanoid receptor 1 (DP1) were lysed and immunoblotted according to 7TM Western Blot Protocol with the phosphorylation-independent anti-DP1 antibody (7TM0388N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 4. Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 5. Immunohistochemical varification of DP1 Prostanoid Receptor antibody in renal cell carcinoma. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0338N). Sections were then incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0338N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation DP1 receptors were uniformly detected at the plasma membrane of nearly all cells.