FP (non-phospho), FP Prostanoid Receptor Antibody

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Preview: Validation of the FP Prostanoid Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of FP Prostanoid Receptor in pancreas

Validation of the FP Prostanoid Receptor in transfected HEK293 cells

Immunohistochemical identification of FP Prostanoid Receptor in pancreas

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Order number: 7TM0344N
Content: 100 µl
Host: Rabbit

The non-phospho-FP Receptor Antibody is directed against the distal end of the... more

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The non-phospho-FP Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human FP. It can be used to detect total FP receptors in Western blots independent of phosphorylation. The non-phospho-FP antibody can also be used to isolate and enrich FP receptors from cell and tissue lysates. It also detects FP in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names PF2AR, PGF receptor, PGF2 alpha receptor, ... more

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Alternative Names	PF2AR, PGF receptor, PGF2 alpha receptor, prostaglandin F receptor
IUPHAR Target ID	344
UniProt ID	P43088
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human FP.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the FP Prostanoid Receptor in transfected HEK293 cells . Native HEK293... more

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Figure 1. Validation of the FP Prostanoid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the FP prostanoid receptor (FP) were lysed and immunoblotted with the phosphorylation-independent anti-FP antibody (7TM0344N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of FP Prostanoid Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FP (non-phospho-FP Prostanoid Receptor) antibody (7TM0344N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.