PAR4 (non-phospho), Proteinase-Activated Receptor 4 Antibody

NEW

Preview: Validation of the Proteinase-Activated Receptor 4 in transfected HEK293 cells

Preview: Immunohistochemical identification of Proteinase-Activated Receptor 4 in small intestine

Validation of the Proteinase-Activated Receptor 4 in transfected HEK293 cells

Immunohistochemical identification of Proteinase-Activated Receptor 4 in small intestine

$ 375.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 5-8 days

Order number: 7TM0350N
Content: 100 µl
Host: Rabbit

The non-phospho-PAR4 receptor antibody is directed against the distal end of the... more

Close menu

The non-phospho-PAR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Proteinase-Activated Receptor 4. It can be used to detect total PAR4 receptors in Western blots independent of phosphorylation. The PAR4 antibody can also be used to isolate and enrich PAR4 receptors from cell and tissue lysates. It also detects PAR4 in cultured cells and tissue sections by immunohistochemistry.

Available downloads:

Alternative Names Coagulation Factor II Receptor-like 3, Protease-Activated... more

Close menu

Alternative Names	Coagulation Factor II Receptor-like 3, Protease-Activated Receptor 4, Thrombin receptor-like 3
IUPHAR Target ID	350
UniProt ID	Q96RI0
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal part of carboxyl-terminal tail of human PAR4.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Proteinase-Activated Receptor 4 in transfected HEK293 cells.... more

Close menu

Figure 1. Validation of the Proteinase-Activated Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Proteinase-Activated Receptor 4 (PAR4) were lysed and immunoblotted with the anti-PAR4 antibody (7TM0350N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Proteinase-Activated Receptor 4 in Small Intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-PAR4 (PAR4 Receptor) antibody (7TM0350N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.