PAR2 (non-phospho), Proteinase-Activated Receptor 2 Antibody

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Preview: Validation of the Proteinase-Activated Receptor 2 in transfected HEK293 cells

Preview: Immunohistochemical identification of Proteinase-Activated Receptor 2 in Kidney

Validation of the Proteinase-Activated Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of Proteinase-Activated Receptor 2 in Kidney

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Order number: 7TM0348N
Content: 100 µl
Host: Rabbit

The non-phospho-PAR2 receptor antibody is directed against the distal end of the... more

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The non-phospho-PAR2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Proteinase-Activated Receptor 2. It can be used to detect total PAR2 receptors in Western blots independent of phosphorylation. The PAR2 antibody can also be used to isolate and enrich PAR2 receptors from cell and tissue lysates. It also detects PAR2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names coagulation factor II receptor-like 1, Protease-activated... more

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Alternative Names	coagulation factor II receptor-like 1, Protease-activated receptor-2
IUPHAR Target ID	348
UniProt ID	P55085
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents part of carboxyl-terminal tail of human PAR2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Proteinase-Activated Receptor 2 in transfected HEK293 cells.... more

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Figure 1. Validation of the Proteinase-Activated Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Proteinase-Activated Receptor 2 (PAR2) were lysed and immunoblotted with the anti-PAR2 antibody (7TM0348N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Proteinase-Activated Receptor 2 in Kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR32 (GPR32 Receptor) antibody (7TM0348N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.