VPAC1 (non-phospho), VIP Receptor 1 Antibody

Preview: Validation of the VIP receptor 1 in transfected HEK293 cells

Preview: Immunocytochemical identification of VIP Receptor 1 in HEK293 cells

Preview: Immunohistochemical identification of VIP Receptor 1 in small intestine

Preview: Immunohistochemical identification of VIP Receptor 1 in enteric ganglia

Validation of the VIP receptor 1 in transfected HEK293 cells

Immunocytochemical identification of VIP Receptor 1 in HEK293 cells

Immunohistochemical identification of VIP Receptor 1 in small intestine

Immunohistochemical identification of VIP Receptor 1 in enteric ganglia

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Order number: 7TM0371N
Content: 100 µl
Host: Rabbit

The VPAC1 antibody is directed against the distal end of the carboxyl-terminal tail of human VIP... more

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The VPAC1 antibody is directed against the distal end of the carboxyl-terminal tail of human VIP Receptor 1. It can be used to detect total VPAC1 receptors in Western blots independent of phosphorylation. The VPAC1 antibody can also be used to isolate and enrich VPAC1 receptors from cell and tissue lysates. It also detects VPAC1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide... more

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Alternative Names	VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide Receptor 1
IUPHAR Target ID	371
UniProt ID	P32241
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide with sequence TRVSPGARRSSSFQAEVSLV corresponds to amino acids 438-457 of human VPAC1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the VIP receptor 1 in transfected HEK293 cells. Native HEK293 cells... more

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Figure 1. Validation of the VIP receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 1 (VPAC1) were lysed and immunoblotted with the anti-VPAC1 antibody (7TM0371N) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of VIP Receptor 1 in HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 1 (VPAC1) were immunocytochemically stained with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:200. Note, VPAC1 receptors were confined to the plasma membrane in transfected cells.

Figure 3. Immunohistochemical identification of VIP Receptor 1 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC1 receptors were uniformly detected at plasma membrane of eptihelial cells.

Figure 4. Immunohistochemical identification of VIP Receptor 1 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC1 receptor staining is absent in muscosa but located in basal portion of the crypts.

Figure 5. Immunohistochemical identification of VIP Receptor 1 in enteric ganglia. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC1 receptors were uniformly detected in nearly all cells of enteric ganglia.

Schulz S, Röcken C, Mawrin C, Weise W, Höllt V, Schulz S. Immunocytochemical identification of... more

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Schulz S, Röcken C, Mawrin C, Weise W, Höllt V, Schulz S. Immunocytochemical identification of VPAC1, VPAC2, and PAC1 receptors in normal and neoplastic human tissues with subtype-specific antibodies. Clin Cancer Res. 2004 Dec 15;10(24):8235-42. PubMed PMID: 15623599.