pS411/pS415-VPAC2 (phospho-VIP Receptor 2 Antibody)

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Preview: Agonist-induced Serine411/Serine415 phosphorylation of VPAC2 Receptor

Preview: Immunocytochemical identification of Serine411/Serine415 phosphorylation of VPAC2-Receptor

Agonist-induced Serine411/Serine415 phosphorylation of VPAC2 Receptor

Immunocytochemical identification of Serine411/Serine415 phosphorylation of VPAC2-Receptor

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Order number: 7TM0372B
Content: 100 µl
Host: Rabbit

Serine411/Serine415 (S411/S415) is a major phosphorylation site of the VPAC2 receptor.... more

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Serine411/Serine415 (S411/S415) is a major phosphorylation site of the VPAC2 receptor. The pS411/pS415-VPAC2 antibody detects phosphorylation in response to high-efficacy agonists. S411/S415 phosphorylation is a key regulator of VPAC2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide... more

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Alternative Names	VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide Receptor 2
IUPHAR Target ID	372
UniProt ID	P41587 (human)
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human VPAC2 around the phosphorylation site Ser411/Ser415.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine411/Serine415 phosphorylation of VPAC2 Receptor. Upper panel,... more

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Figure 1. Agonist-induced Serine411/Serine415 phosphorylation of VPAC2 Receptor. Upper panel, HEK293 cells stably expressing the VPAC2-Receptor (VPAC2) were either not exposed or exposed to 1 µM of specific VPAC-Receptor agonist PACAP-27 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS411/pS415-VPAC2 antibody (7TM0372B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-VPAC2 antibody (7TM0372N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Immunocytochemical identification of Serine411/Serine415 phosphorylation of VPAC2-Receptor. HEK293 cells stably expressing the VPAC2-Receptor (VPAC2) were either not exposed or exposed to 1 µM of specific VPAC-Receptor agonist PACAP-27 and immunocytochemically stained with the anti-pS411/pS415-VPAC2 antibody (7TM0372B) at a dilution of 1:200. Note, Serine411/Serine415-phosphorylated VPAC2 receptors were not detectable in untreated cells (0 min). Serine411/Serine415-phosphorylated VPAC2 receptors were seen at the plasma membrane after 5 min PACAP-27 stimulation.