pT429/pS431/pT432-VPAC1 (phospho-VIP Receptor 1 Antibody)

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Preview: Agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1 Receptor

Preview: Immunocytochemical identification of agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1 Receptor

Agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1 Receptor

Immunocytochemical identification of agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1 Receptor

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Order number: 7TM0371B
Content: 100 µl
Host: Rabbit

Threonine429/Serine431/Threonine432 (T429/S431/T432) is a major phosphorylation site of the... more

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Threonine429/Serine431/Threonine432 (T429/S431/T432) is a major phosphorylation site of the VPAC1 receptor. The pT429/pS431/pT432-VPAC1 antibody detects phosphorylation in response to high-efficacy agonists. T429/S431/T432 phosphorylation is a key regulator of VPAC1 desensitization, β-arrestin recruitment and internalization.

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Alternative Names VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide... more

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Alternative Names	VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide Receptor 1
IUPHAR Target ID	371
UniProt ID	P32241
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human VPAC1 around the phosphorylation site Thr429/Ser431/Thr432.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1... more

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Figure 1. Agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1 Receptor. Upper panel, HEK293 cells stably expressing the VPAC1-Receptor (VPAC1) were either not exposed or exposed to 1 µM of specific VPAC1-Receptor agonist PACAP-27 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT429/pS431/pT432-VPAC1 antibody (7TM0371B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-VPAC1 antibody (7TM0371N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Immunocytochemical identification of agonist-induced Threonine429/Serine431/Threonine432 phosphorylation of VPAC1 Receptor. HEK293 cells stably expressing the VPAC1 Receptor (VPAC1) were either not exposed or exposed to 1 μM of specific VPAC1-Receptor agonist PACAP-2 for 30 min and immunocytochemically stained with anti-VPAC1 (VPAC1 Receptor) antibody (7TM0371B) at a dilution of 1:200. Note, Threonine429/Serine431/Threonine432-phosphorylated VPAC1 receptors were not detectable in untreated cells (0 min). Threonine429/Serine431/Threonine432-phosphorylated VPAC1 receptors were seen at the plasma membrane and perinuclear clusters of vesicles after 30 min PACAP-27 treatment.