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pS422/pS425-VPAC1 (phospho-VIP Receptor 1 Antibody)

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  • 7TM0371A
  • 100 µl
  • Rabbit
Serine422/Serine425 (S422/S425) is a major phosphorylation site of the VPAC1 receptor.... more

Serine422/Serine425 (S422/S425) is a major phosphorylation site of the VPAC1 receptor. The pS422/pS425-VPAC1 antibody detects phosphorylation in response to high-efficacy agonists. S422/S425 phosphorylation is a key regulator of VPAC1 desensitization, β-arrestin recruitment and internalization.

  Alternative Names VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide... more

 

Alternative Names VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide Receptor 1
IUPHAR Target ID  371
UniProt ID  P32241
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human VPAC1 around the phosphorylation site Ser422/Ser425.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine422/Serine425 phosphorylation of VPAC1 Receptor. Upper panel,... more

Figure 1. Agonist-induced Serine422/Serine425 phosphorylation of VPAC1 Receptor. Upper panel, HEK293 cells stably expressing the VPAC1-Receptor (VPAC1) were either not exposed or exposed to 1 µM of specific VPAC1-Receptor agonist PACAP-27 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS422/pS425-VPAC1 antibody (7TM0371A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-VPAC1 antibody (7TM0371N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Immunocytochemical identification of agonist-induced Serine422/Serine425 phosphorylation of VPAC1 Receptor. HEK293 cells stably expressing the VPAC1 Receptor (VPAC1) were either not exposed or exposed to 1 μM of specific VPAC1-Receptor agonist PACAP-2 for 30 min and immunocytochemically stained with anti-VPAC1 (VPAC1 Receptor) antibody (7TM0371A) at a dilution of 1:200. Note, Serine422/Serine425-phosphorylated VPAC1 receptors were not detectable in untreated cells (0 min). Serine422/Serine425-phosphorylated VPAC1 receptors were seen at the plasma membrane and perinuclear clusters of vesicles after 30 min PACAP-27 treatment.

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