C5a1 (non-phospho), Complement C5a Receptor 1 Antibody

Figure 1. Validation of the Complement C5a Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the complement C5a receptor 1 (C5a1) were lysed and immunoblotted with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of Complement C5a Receptor 1 in HEK293 cells. HEK293 cells stably expressing the complement C5a receptor 1 (C5a1) were either not exposed or exposed to 1 μM C5a (complement fragment 5a) for 30 min and immunocytochemically stained with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM0032N-WB) at a dilution of 1:200. Note, C5a1 receptors were confined to the plasma membrane in untreated cells (0 min). C5a1 receptors were seen in perinuclear clusters of vesicles after 30 min C5a1 exposure.

Figure 3. Immunohistochemical identification of Complement C5a receptor 1 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM0032N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were detected at the plasma membrane of a distinct population of cells in human spleen.

Figure 4. Immunohistochemical identification of Complement C5a receptor 1 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM0032N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were detected at the plasma membrane of a distinct population of cells in human spleen.

Figure 5. Immunohistochemical identification of Complement C5a Receptor 1 in human lung. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM00032N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were uniformly detected at the plasma membrane of lung macrophages.

Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PLoS One. 2021 Feb 19;16(2):e0246939. doi: 10.1371/journal.pone.0246939. PMID: 33606748; PMCID: PMC7894821.