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C5a2 (non-phospho), Complement C5a Receptor 2 Antibody

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  • 7TM0033N
  • 100 µl
  • Rabbit
The non-phospho-C5a2 receptor antibody is directed against the distal part of the... more

The non-phospho-C5a2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human C5a2. It can be used to detect total C5a2 receptors in Western blots independent of phosphorylation. The non-phospho-C5a2 antibody can also be used to isolate and enrich C5a2 receptors from cell and tissue lysates.

  Alternative Names C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin... more

 

Alternative Names C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin Chemotactic Receptor 2
IUPHAR Target ID  33
UniProt ID  Q9P296
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents carboxyl-terminal tail of human C5a2
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Complement C5a Receptor 2 in transfected HEK293 cells. Native... more

Figure 1. Validation of the Complement C5a Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Complement C5a Receptor 2 (C5a2) were lysed and immunoblotted with the phosphorylation-independent anti-C5a2 antibody (7TM0033N-WB) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of the Complement C5a Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Complement C5a Receptor 2 (C5a2) were immunocytochemically stained with the phosphorylation-independent anti-C5a2 antibody (7TM0033N-WB) at a dilution of 1:200.

Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical... more

Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PLoS One. 2021 Feb 19;16(2):e0246939. doi: 10.1371/journal.pone.0246939. PMID: 33606748; PMCID: PMC7894821.

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