7TM GPR75 Stable Cell Line

7TM GPR75 Stable Cell Line. 7TM stable cell lines are shipped on dry ice.

Figure 1. Immuncytochemical verification of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged metabotropic Glutamate Receptor 2 (HA-mGLU2), HA-tagged Calcitonin Receptor-like Receptor (HA-CALCRL), HA-tagged Corticotropin-releasing Factor Receptor 1 (HA-CRF1), HA-tagged orphan G Protein-coupled Receptor 20 (HA-GPR20), metabotropic Glutamate Receptor 4 (HA-mGLU4) or HA-tagged Dopamine receptor 1 (HA-D1) were immunocytochemically stained with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:200. Note, the receptors are located at the plasma membrane of the cells.

Figure 2. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged C-C chemokine receptor 5 (HA-CCR5), HA-tagged C-C chemokine receptor 7 (HA-CCR7), HA-tagged C-C chemokine receptor 8 (HA-CCR8), HA-tagged C-C chemokine receptor 9 (HA-CCR9), HA-tagged C-C chemokine receptor 10 (HA-CCR10), HA-tagged muscarinic acetylcholine receptor 3 (HA-M3), HA-tagged histamine H1 receptor (HA-H1), HA-tagged C-X3-C motif chemokine receptor 1 (HA-CX3CR1) or HA-tagged hypocretin receptor 1/orexin receptor 1 (HA-OX1) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 3. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Adenosin Diphosphate Receptor 4 (HA-P2Y4), HA-tagged Adenosin Diphosphate Receptor 12 (HA-P2Y12), HA-tagged Alpha-1 Adrenergic Receptor A (HA-α1A), HA-tagged Dopamine Receptor 5 (HA-D5) or HA-tagged Histamine H2 receptor (HA-H2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 4. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Hypocretin Receptor 1/Orexin Receptor 1 (HA-OX1), HA-tagged Canabinoid Receptor 1 (HA-CB1), HA-tagged Canabinoid Receptor 1 (HA-CB2), HA-tagged Galanin Receptor 1  (HA-GAL1) or HA-tagged G Protein-coupled Estrogen Receptor  (HA-GPER) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

Figure 5. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Serotonin Receptor 4 (HA-5-HT4), HA-tagged Serotonin Receptor 6 (HA-5-HT6), HA-tagged Serotonin Receptor 2A (HA-5-HT2A), HA-tagged Cholecystokinin Receptor 1  (HA-CCK1) or HA-tagged Cholecystokinin Receptor 2  (HA-CCK2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.

7TM Stable Cell Lines are available for all our 7TM Antibodies. Please inquire for all other GPCRs. Delivery time for stable cell lines is 10 to 14 days.