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- Order number: 7TM0099N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR32 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR32. It can be used to detect total GPR32 receptors in Western blots independent of phosphorylation. The GPR32 antibody can also be used to isolate and enrich GPR32 receptors from tissue lysates. It also detects GPR32 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | RvD1, RVDR1, resolvin D1 receptor |
| IUPHAR Target ID | 99 |
| Uniprot ID | O75388 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR32. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the G protein-coupled receptor 32 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G protein-coupled receptor 32 (GPR32) were lysed and immunoblotted with the phosphorylation-independent anti-GPR32 antibody (7TM0099N-IC) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of GPR32 Receptor in Pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR32 (GPR32 Receptor) antibody (7TM0099N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.
Figure 3. Immunohistochemical identification of GPR32 Receptor in Small Intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR32 (GPR32 Receptor) antibody (7TM0099N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.