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NPB (IHC-grade), Neuropeptide B Antibody

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  • 7TM0NPB-IC
  • 100 µl
  • Rabbit
The NPB antibody is directed against human Neuropeptide B. The NPB antibody can be used to... more

The NPB antibody is directed against human Neuropeptide B. The NPB antibody can be used to detect Neuropeptide B in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.

  Alternative Names  NPB, neuropeptide B IUPHAR Target ID... more

 

Alternative Names  NPB, neuropeptide B
IUPHAR Target ID  1502
UniProt ID  Q8NG41
Immunohistochemistry (IHC)
1:100
Western Blot (WB)  -
Species Reactivity   Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents human NPB.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification of Neuropeptide B (NPB) in rat hypothalamus.... more

Figure 1. Immunohistochemical identification of Neuropeptide B (NPB) in rat hypothalamus. Rats were treated with an intracerebroventricular injection with 100 µg colchicine (10µg/µl) followed by vascular perfusion and fixation. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NPB (Neuropeptide B) antibody (7TMNPB-IC) at a dilution of 1:100 or incubated with the same antibody in the same dilution but antibody was preincubated with 10 µg/ml Neuropeptide B (right picture). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation with streptavidine-cyanine 3.18. Note, after colchicine treatement Neuropeptide B was located in neuronal somata. NPB staining was abolished in section when antibody was preincubated with NPB peptide.

Figure 2. Immunohistochemical identification of Neuropeptide B (NPB) in rat hypothalamus. Rats were treated with an intracerebroventricular injection of a vehicle instead of colchicine (Figure 1) followed by vascular perfusion and fixation. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NPB (Neuropeptide B) antibody (7TMNPB-IC) at a dilution of 1:100 or incubated with the same antibody in the same dilution but antibody was preincubated with 10 µg/ml Neuropeptide B (right picture). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation with streptavidine-cyanine 3.18. Note, without colchicine treatment Neuropeptide B was located in fibers and terminals. NPB staining was abolished in section when antibody was preincubated with NPB peptide.

 

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