mPAC1 (IHC-grade), PACAP Receptor 1 Antibody (Mouse)

Figure 1. Immunohistochemical identification of PACAP Receptor 1 in mouse spinal cord dorsal horn. Brain sections were dewaxed, microwaved in citric acid, and incubated with anti-PAC1 (PACAP receptor 1) antibody (7TM0370MN-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, PAC1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 2. Immunocytochemical identification of PACAP Receptor 1 in HEK293 cells. HEK293 cells stably expressing the PACAP Receptor 1 (PAC1) were either not exposed or exposed to 1 μM PACAP (pituitary adenylate cyclase-activating peptide)) for 30 min and immunocytochemically stained with anti-PAC1 (PACAP receptor 1) antibody (7TM0370MN-IC) at a dilution of 1:200. Note, PAC1 receptors were confined to the plasma membrane in untreated cells (0 min). PAC1 receptors were seen in perinuclear clusters of vesicles after 30 min PACAP exposure.

Figure 3. Validation of the PACAP Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the mouse PACAP Receptor 1 (PAC1) were lysed and immunoblotted with the phosphorylation-independent anti-PAC1 antibody (7TM0370MN-IC) at a dilution of 1:1000.

Liu NJ, Schnell SA, Schulz S, Wessendorf MW, Gintzler AR. Regulation of spinal dynorphin 1-17 release by endogenous pituitary adenylyl cyclase-activating polypeptide in the male rat: relevance of excitation via disinhibition. J Pharmacol Exp Ther. 2011 Feb;336(2):328-35. doi: 10.1124/jpet.110.173039. Epub 2010 Oct 25. PubMed PMID: 20974701; PubMed Central PMCID: PMC3033715.