pS412-β1 (phospho-β1-Adrenoceptor Antibody)

NEW

Agonist-induced Serine412 phosphorylation of the β1-Adrenoceptor

$ 375.00 *

Ready to ship today,
Delivery time appr. 5-8 days

Order number: 7TM0028B
Content: 100 µl
Host: Rabbit

Serine412 (S412) is a major phosphorylation site of the β1 adrenoceptor. The pS412-β1... more

Serine412 (S412) is a major phosphorylation site of the β1 adrenoceptor. The pS412-β1 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S412 phosphorylation is primarily mediated by GRKs and is a key regulator of β1 desensitization, β-arrestin recruitment and internalization.

Available downloads:

Alternative Names β1, B1AR, ADRB1R, β1 Adrenergic... more

Close menu

Alternative Names	β1, B1AR, ADRB1R, β1 Adrenergic Receptor, β1-Adrenoceptor
IUPHAR Target ID	28
UniProt ID	P08588
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human β1 around the phosphorylation site of Ser412.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine412 phosphorylation of the β1-Adrenoceptor. Upper... more

Close menu

Figure 1. Agonist-induced Serine412 phosphorylation of the β1-Adrenoceptor. Upper panel, HEK293 cells stably expressing the β1-Adrenoceptor (β1) were either not exposed or exposed to 10 µM of specific β1-adrenoceptor agonist Norepinephrene for 30 minutes. Cells were lysed and immunoblotted with the anti-pT404-β1 antibody (7TM0028B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-β1 antibody (7TM0028N) at a dilution of 1:1000 to confirm equal loading of the gel.

Be the first to decipher the β1 phosphorylation barcode and let us know. more

Close menu

Be the first to decipher the β1 phosphorylation barcode and let us know.

Close menu