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- Order number: 7TM0032E
- Content: 100 µl
- Host: Rabbit
Threonine342 (T342) is a major phosphorylation site of the C5a1 receptor. The pT342-C5a1 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. T342 phosphorylation is a key regulator of C5a1 desensitization, β-arrestin recruitment and internalization.
Alternative Names | C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin Chemotactic Receptor 1 |
IUPHAR Target ID | 32 |
UniProt ID | P21730 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | 1:200 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human C5a1 around the phosphorylation site of Thr342. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine342 phosphorylation of the Complement C5a Receptor 1. Upper panel, HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were either not exposed or exposed to 1 µM specific Complement C5a receptor agonist CO28 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT342-C5a1 antibody (7TM0032E) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.
Figure 2. Analysis of dose-dependent Complement C5a Receptor 1 phosphorylation using three phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were either not exposed or exposed to decreasing concentrations of specific Complement C5a receptor agonist CO28 ranging from 100 µM to 10 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT336-C5a1 antibody (7TM0032C) or anti-pS338/pT339-C5a1 antibody (7TM0032D) or anti-pT342-C5a1 antibody (7TM0032E) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.
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