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- Order number: 7TM0215N
- Content: 100 µl
- Host: Rabbit
The non-phospho-D2 receptor antibody is directed against the third intracellular loop of mouse, rat and human D2 dopamine receptor. It detects both the long and short form of D2. It can be used to detect total D2 receptors in Western blots independent of phosphorylation. The non-phospho-D2 antibody can also be used to isolate and enrich D2 receptors from brain lysates. It also detects D2 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | D2, D2R, Dopamine Receptor 2 |
| IUPHAR Target ID | 215 |
| UniProt ID | P14416 (human) P61168 (mouse) P61169 (rat) |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | 1:200 |
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents carboxyl-terminal tail of human D2 |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the Dopamine Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were lysed and immunoblotted with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of Dopamine Receptor 1 and Dopamine Receptor 2 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-IC) at a dilution of 1:100 (left panel) or incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100 (right panel). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 3. Immunohistochemical identification of Dopamine Receptor 2 in hypothalamus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected on a distinct population of cells in the neuroendocrine tumor.
Figure 4. Immunohistochemical identification of Dopamine Receptor 2 in human small cell lung carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected on a distinct population of cells in the small cell lung carcinoma.
Figure 5. Immunohistochemical identification of Dopamine Receptor 2 in human pancreatic islets. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected in pancreatic islets.
Figure 6. Immunocytochemical identification of Dopamine Receptor 2 in transfected HEK293 cells. HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were either not exposed or exposed to 10 μM Quinpirole for 30 min and immunocytochemically stained with phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:200. Note, D2 receptors were confined to the plasma membrane in untreated cells (0 min). D2 receptors were seen at plasma membrane and in perinuclear clusters of vesicles after 30 min Quinpirole exposure.
Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling. Sci Rep. 2021 Apr 15;11(1):8288. doi: 10.1038/s41598-021-87417-2. PMID: 33859231; PMCID: PMC8050214.
