SST5 (non-phospho), Somatostatin Receptor 5 Antibody

Figure 1. Validation of the Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were lysed and immunoblotted with the phosphorylation-independent anti-SST5 antibody (7TM0359N-WB) at a dilution of 1:1000.

Figure 2. Western blot analysis of Somatostatin Receptor 5 in neuroendocrine tumors. Human tumor samples (T1, T2) from neuroendocrine tumors were homogenated and immunoblotted using anti-SST5 (non-phospho-Somatostatin-Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:1000.

Figure 3. Immunocytochemical identification of Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor (SST5) were immunocytochemically stained with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:200. Note, SST5 receptors were confined to the plasma membrane in tranfected cells.

Figure 4. Immunohistochemical identification of Somatostatin Receptor 5 in human pancreatic islets. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected at the plasma membrane of nearly all cells in human pancreatic islets.

Figure 5. Immunohistochemical identification of Somastatin Receptor 5 in human anterior pituitary. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected on a distinct population of cells in the anterior pituitary.

Figure 6. Immunohistochemical identification of Somastatin Receptor 5 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 7. Immunohistochemical identification of Somastatin Receptor 5 in human pheochromocytoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all cells in pheochromocytoma.

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