pT329/pT330-BLT2 (phospho-Leukotriene Receptor BLT2 Antibody)

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Order number: 7TM0268B
Content: 100 µl
Host: Rabbit

Threonine329/Threonine330 (T329/T330) is a major phosphorylation site of the BLT2 receptor.... more

Threonine329/Threonine330 (T329/T330) is a major phosphorylation site of the BLT2 receptor. The pT329/pT330-BLT2 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T329/T330 phosphorylation is a key regulator of BLT2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names BLTR2, LTB4 receptor JULF2, 12-HHT receptor IUPHAR... more

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Alternative Names	BLTR2, LTB4 receptor JULF2, 12-HHT receptor
IUPHAR Target ID	268
UniProt ID	Q9NPC1
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human BLT2 around the phosphorylation site of Thr329/Thr330.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine329/Threonine330 phosphorylation of the Leukotriene... more

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Figure 1. Agonist-induced Threonine329/Threonine330 phosphorylation of the Leukotriene Receptor BLT2. Upper panel, HEK293 cells stably expressing the Leukotriene Receptor BLT2 (BLT2) were either not exposed or exposed to 100 nM specific Leukotriene receptor BLT2 agonist LTB4 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT329/pT330-BLT2 antibody (7TM0268B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-BLT2 antibody to confirm equal loading of the gel.

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