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pS369/pS374-NPY2 (phospho-Neuropeptide Y Receptor 2 Antibody)

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  • 7TM0306C
  • 100 µl
  • Rabbit
Serine369/Serine374 (S369/S374) is a major phosphorylation site of the Neuropeptide Y Receptor... more

Serine369/Serine374 (S369/S374) is a major phosphorylation site of the Neuropeptide Y Receptor 2. The pS369/pS374-NPY2 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S369/S374 phosphorylation is primarily mediated by GRKs and is a key regulator of NPY2 desensitization, β-arrestin recruitment and internalization.

  Alternative Names Y2, NPY 2 receptor, neuropeptide Y receptor type 2... more

 

Alternative Names Y2, NPY 2 receptor, neuropeptide Y receptor type 2
IUPHAR Target ID  306
UniProt ID  P49146
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human Y2 around the phosphorylation site of Ser351/Ser354.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine369/Serine374 phosphorylation of the Neuropeptide Y receptor 2.... more

Figure 1. Agonist-induced Serine369/Serine374 phosphorylation of the Neuropeptide Y receptor 2. Upper panel, HEK293 cells stably expressing the NPY2-Receptor (NPY2) were either not exposed or exposed to 1 µM of  NPY2 receptor agonist Neuropeptide Y for 30 minutes. Cells were lysed and immunoblotted with the anti-pS369/pS374-NPY2 antibody (7TM0306C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NPY2 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent NPY2 receptor phosphorylation using a panel of phosphosite-specific antibodies. Upper four panels, HEK293 cells stably expressing the NPY2 receptor (NPY2) were either not exposed or exposed to increasing concentrations of Neuropeptide Y ranging from 100 pM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS351/pS354-NPY2 antibody (7TM0306A) or anti-pS354/pT356-NPY2 antibody (7TM0306B) or anti-pS369/pS374-NPY2 antibody (7TM0306C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NPY2 antibody to confirm equal loading of the gel.

Be the first to decipher the NPY2 phosphorylation barcode and let us know. more

Be the first to decipher the NPY2 phosphorylation barcode and let us know.

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