OX2 (non-phospho) Orexin Receptor 2 Antibody

Figure 1. Validation of the Orexin Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Orexin Receptor 2 (OX2) were lysed and immunoblotted with the anti-OX2 antibody (7TM0322N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Orexin Receptor 2 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-OX2 (non-phospho-Orexin Receptor 2) antibody (7TM0322N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical varification of Orexin Receptor 2 antibody in pancreas. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-OX2 (non-phospho-Orexin Receptor 2) antibody (7TM0322N). Sections were then incubated with anti-OX2 (non-phospho-Orexin Receptor 2) antibody (7TM0322N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation OX2 receptors were uniformly detected at the plasma membrane of nearly all cells.

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