pT358/pS360-GPR132 (phospho-GPR132 Antibody)

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Agonist-induced Threonine358/Serine360 phosphorylation of GPR132

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Order number: 7TM0128C
Content: 100 µl
Host: Rabbit

Threonine358/Serine360 (T358/S360) is major phosphorylation site of human GPR132 receptor. The... more

Threonine358/Serine360 (T358/S360) is major phosphorylation site of human GPR132 receptor. The pT358/pS360-GPR132 antibody detects phosphorylation in response to agonists. T358/S360 phosphorylation is likely to be involved in efficient ligand sequestration by GPR132.

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Alternative Names G Protein-coupled Receptor G2A IUPHAR Target ID... more

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Alternative Names	G Protein-coupled Receptor G2A
IUPHAR Target ID	128
UniProt ID	Q9UNW8
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR132 around the phosphorylation site of Thr358/Ser360.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine358/Serine360 phosphorylation of GPR132. Upper... more

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Figure 1. Agonist-induced Threonine358/Serine360 phosphorylation of GPR132. Upper panel, HEK293 cells transiently expressing the human GPR132-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR132-Receptor agonist 9-HODE for 5 minutes. Cells were lysed and immunoblotted with the anti-pT358/pS360-GPR132 antibody (7TM0128C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GRP132 antibody to confirm equal loading of the gel.

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