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- Order number: 7TM0210N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR158/mGlyR receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR158/mGlyR. It can be used to detect total GPR158/mGlyR receptors in Western blots independent of phosphorylation. The GPR158/mGlyR antibody can also be used to isolate and enrich GPR158/mGlyR receptors from tissue lysates.
| Alternative Names | |
| IUPHAR Target ID | 210 |
| Uniprot ID | Q5T484 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR158/mGlyR. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the GPR158/mGlyR Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR158/mGlyR Receptor (GPR158/mGlyR) were lysed and immunoblotted with the phosphorylation-independent anti-GPR158/mGlyR antibody (7TM0210N) at a dilution of 1:1000
Figure 2. Immunohistochemical identification of GPR158/mGlyR Receptor in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR158/mGlyR (GPR158/mGlyR Receptor) antibody (7TM0210N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin
Figure 3. Immunohistochemical identification of GPR158/mGlyR Receptor in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR158/mGlyR (GPR158/mGlyR Receptor) antibody (7TM0210N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin