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- Order number: 7TM0102B
- Content: 100 µl
- Host: Rabbit
Serine298/Serine301 (S298/S301) is a major phosphorylation site of the mouse GPR35 receptor. The pS298/pS301-mGPR35 antibody detects phosphorylation in response to high-efficacy agonists. S298/S301 phosphorylation is a key regulator of mouse GPR35 desensitization, β-arrestin recruitment and internalization.
| Alternative Names | GPR35, G-protein coupled Receptor 35 |
| IUPHAR Target ID | 102 |
| UniProt ID | Q9ES90 |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | 1:200 |
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from mouse GPR35 around the phosphorylation site Ser298/Ser301. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine298/Serine301 phosphorylation of mouse GPR35 Receptor. Upper panel, HEK293 cells transiently expressing the eYFP-tagged mouse GPR35-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR35-Receptor agonist zaprinast for 5 minutes. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the anti-pS298/pS301-mGPR35 antibody (7TM0102B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.
Figure 2. Immunocytochemical Identification of Serine298/Serine301 phosphorylation of mouse GPR35 Receptor. Flp-ln TREx 293 cells stably expressing hGPR35a-HA were either treated with vehicle or 100 μM zaprinast for 5 min and immunocytochemically stained with anti-pS298/pS301-mGPR35 antibody (7TM0102A) at a dilution of 1:200.
Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA, Milligan G. Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor. J Biol Chem. 2022 Mar;298(3):101655. doi: 10.1016/j.jbc.2022.101655. Epub 2022 Jan 29. PMID: 35101446; PMCID: PMC8892012.
Ganguly A, Quon T, Jenkins L, Joseph B, Al-Awar R, Chevigne A, Tobin AB, Uehling DE, Hoffmann C, Drube J, Milligan G. G protein-receptor kinases 5/6 are the key regulators of G protein-coupled receptor 35-arrestin interactions. J Biol Chem. 2023 Oct;299(10):105218. doi: 10.1016/j.jbc.2023.105218. Epub 2023 Sep 1. PMID: 37660910; PMCID: PMC10520886.
