pS377-QRFPR (phospho-QRFP Receptor Antibody)

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Agonist-induced Serine377 phosphorylation of QRFP Receptor

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Order number: 7TM0333B
Content: 100 µl
Host: Rabbit

Serine377 (S377) is a major phosphorylation site of the QRFP receptor. The pS377-QRFPR... more

Serine377 (S377) is a major phosphorylation site of the QRFP receptor. The pS377-QRFPR antibody detects phosphorylation in response to high-efficacy agonists. S377 phosphorylation is a key regulator of QRFPR desensitization, β-arrestin recruitment and internalization.

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Alternative Names GPR103 | G protein-coupled receptor... more

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Alternative Names	GPR103 \| G protein-coupled receptor 103 \| peptide p518 receptor
IUPHAR Target ID	333
UniProt ID	Q96P65
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human QRFPR around the phosphorylation site Ser377.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine377 phosphorylation of QRFP Receptor. Upper panel, HEK293... more

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Figure 1. Agonist-induced Serine377 phosphorylation of QRFP Receptor. Upper panel, HEK293 cells stably expressing the QRFP-Receptor (QRFPR) were either not exposed or exposed to 1 µM of specific QRFP-Receptor agonist QRFP26 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS377-QRFPR antibody (7TM0333B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-QRFPR antibody to confirm equal loading of the gel.

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