pS358/pS360-GPR52 (phospho-GPR52 Antibody)

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Agonist-induced Serine358/Serine360 phosphorylation of the GPR52 Receptor

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Order number: 7TM0108A
Content: 100 µl
Host: Rabbit

Serine358/Serine360 (S358/S360) is major phosphorylation site of the GPR52 Receptor (GPR52). The... more

Serine358/Serine360 (S358/S360) is major phosphorylation site of the GPR52 Receptor (GPR52). The pS358/pS360-GPR52 antibody detects phosphorylation in response to agonists. S358/S360 phosphorylation is likely to be involved in efficient ligand sequestration.

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Alternative Names IUPHAR Target ID 108 Uniprot... more

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Alternative Names
IUPHAR Target ID	108
Uniprot ID	Q9Y2T5
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR52 around the phosphorylation site of Ser358/Ser360.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine358/Serine360 phosphorylation of the GPR52 Receptor. Upper... more

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Figure 1. Agonist-induced Serine358/Serine360 phosphorylation of the GPR52 Receptor. Upper panel, HEK293 cells stably expressing the GPR52 Receptor (GPR52) and transiently GRK2 (Ratio GPR52 receptor:GRK2 = 10:1) were preincubated with serum-free media. After 2 hours cells were either not exposed or exposed to 100 nM FTBMT for 30 minutes. Cells were lysed and immunoblotted with the anti-pS358/pS360-GPR52 antibody (7TM0108A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GPR52 (7TM0108N) antibody to confirm equal loading of the gel.

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