pT315-GPR139 (phospho-GPR139 Antibody)

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Agonist-induced Threonine315 phosphorylation of the GPR139 Receptor

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Order number: 7TM0130A
Content: 100 µl
Host: Rabbit

Threonine315 (T315) is major phosphorylation site of the GPR139 Receptor (GPR139). The... more

Threonine315 (T315) is major phosphorylation site of the GPR139 Receptor (GPR139). The pT315-GPR139 antibody detects phosphorylation in response to agonists. T315 phosphorylation is likely to be involved in efficient ligand sequestration.

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Alternative Names GPRG1, G-protein-coupled receptor PGR3 IUPHAR... more

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Alternative Names	GPRG1, G-protein-coupled receptor PGR3
IUPHAR Target ID	130
Uniprot ID	Q6DWJ6
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR139 around the phosphorylation site of Thr315.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine315 phosphorylation of the GPR139 Receptor. Upper panel,... more

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Figure 1. Agonist-induced Threonine315 phosphorylation of the GPR139 Receptor. Upper panel, HEK293 cells stably expressing the GPR139 Receptor (GPR139) and transiently GRK2 (Ratio GPR139 receptor:GRK2 = 10:1) were preincubated with serum-free media. After 2 hours cells were either not exposed or exposed to 100 nM JNJ-63533054 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT315-GPR139 antibody (7TM0130A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GPR139 antibody to confirm equal loading of the gel.

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