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- Order number: 7TM0125N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR101 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR101. It can be used to detect total GPR101 receptors in Western blots independent of phosphorylation. The GPR101 antibody can also be used to isolate and enrich GPR101 receptors from tissue lysates. It also detects GPR101 in cultured cells and tissue sections by immunohistochemistry
| Alternative Names | Sphingosine 1-phosphate receptor |
| IUPHAR Target ID | 85 |
| Uniprot ID | P46095 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR6. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the GPR6 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR6 Receptor (GPR6) were lysed and immunoblotted with the phosphorylation-independent anti-GPR6 antibody (7TM0085N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of GPR6 Receptor in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR6 (GPR6 Receptor) antibody (7TM0085N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.