ETA (IHC-grade), Endothelin Receptor A Antibody

ETA (IHC-grade), Endothelin Receptor A Antibody

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Order number: 7TM0219N-IC
Content: 100 µl
Host: Rabbit

The ETA receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The ETA receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human endothelin receptor A. It can be used to detect ETA receptors in Western blots in a phosphorylation-sensitive manner phosphorylation. After agonist activation, ETA is phosphorylated at the carboxyl-terminus. Because the ETA antibody detects the same epitope, it no longer binds to the receptor. It detects selectively ETA receptors that have been hot activated and not phosphorylated. The ETA antibody can also be used to isolate and enrich ETA receptors from cell and tissue lysates. It also detects ETA in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names ETA, EDNRA, Endothelin Receptor A IUPHAR Target ID... more

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Alternative Names	ETA, EDNRA, Endothelin Receptor A
IUPHAR Target ID	219
UniProt ID	P25101 (human) Q61614 (mouse) P26684 (rat)
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide with sequence KNHDQNNHNTDRSSHKDSMN corresponds to amino acids 408-427 in human, mouse and rat ETA
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Endothelin Receptor A in mouse heart. Sections... more

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Figure 1. Immunohistochemical identification of Endothelin Receptor A in mouse heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ETA (Endothelin receptor A) antibody (7TM0219N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ETA receptors were detected at the plasma membrane of cardiomyocytes.

Figure 2. Validation of the Endothelin Receptor A in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Endotheline Receptor A (ETA) were lysed and immunoblotted with the phosphorylation-independent anti-ETA antibody (7TM0219N) at a dilution of 1:1000.

Lupp A, Mann A, Heeb A, Kaemmerer D, Sänger J, Evert M, Evert K, Mawrin C, Schulz S.... more

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Lupp A, Mann A, Heeb A, Kaemmerer D, Sänger J, Evert M, Evert K, Mawrin C, Schulz S. Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8. Peptides. 2015 Apr;66:19-25. doi: 10.1016/j.peptides.2015.02.005. Epub 2015 Feb 20. PubMed PMID: 25708416.

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