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- Order number: 7TM0226B
- Content: 100 µl
- Host: Rabbit
Threonine306/Threonine310 (T306/T310) is major phosphorylation site of the FFA 2 Receptor (FFA2). The pT306/pT310-FFA2 antibody detects phosphorylation in response to agonists. T306/T310 phosphorylation is likely to be involved in efficient ligand sequestration by FFA2.
Alternative Names | FFAR2, Free Fatty Acid Receptor 2, GPCR3 |
IUPHAR Target ID | 226 |
UniProt ID | O15552 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | 1:200 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human FFA2 around the phosphorylation site of Thr306/Thr310. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine306/Threonine310 phosphorylation of the FFA2 Receptor. Upper panel, parental or Flp-In TREx 293 harbouring human FFA2-eYFP induced to express the receptor construct (+ dox) were treated with vehicle or 2mM propanoic acid (C3) for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pT306/pT310-FFA2 antibody (7TM0226B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.
Figure 2. Immunocytochemical identifiction of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2. Flp-In TREx 293 cells stably expressing hFFA2-HA were either treated with vehicle or 2 mM C3 for 2 min and immunocytochemically stained with the anti-pT306pT310-FFA2 antibody (7TM0226B) at a dilution of 1:200. Note, Threonine306/Threonine310-phosphorylated FFA2 receptors were not detectable in untreated cells (vehicle). Threonine306/Threonine310-phosphorylated FFA2 receptors were seen at the plasma membrane after 2 min C3-treatment.