5-HT2C (IHC-grade), 5-HT Receptor 2C Antibody

Figure 1. Immunohistochemical varification of 5-Hydroxytryptamine Receptor 2C antibody in Rat Striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-5HT2C (5-Hydroxytryptamine Receptor 2C) antibody (7TM0008N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Immunohistochemical varification of 5-Hydroxytryptamine Receptor 2C antibody in Mouse Striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-5HT2C (5-Hydroxytryptamine Receptor 2C) antibody (7TM0008N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Immunohistochemical varification of 5-Hydroxytryptamine Receptor 2C antibody in Mouse Striatum. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-5-HT2C (non-phospho-5-HT2C Receptor) antibody (7TM0008N-IC). Sections were then incubated with anti-5-HT2C (non-phospho-5-Hydroxytryptamine Receptor 2C) antibody (7TM0008N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation 5-HT2C receptors were uniformly detected at the plasma membrane of nearly all cells.