pS354/pT355-5-HT4 (phospho-5-HT Receptor 4 Antibody)

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Agonist-induced Serine354/Threonine355 phosphorylation of the 5-Hydroxytryptamine Receptor 4

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Order number: 7TM0009C
Content: 100 µl
Host: Rabbit

Serine354/Threonine355 is a major phosphorylation site of the 5-HT4 receptor.... more

Serine354/Threonine355 is a major phosphorylation site of the 5-HT4 receptor. The pS354/pT355-5-HT4 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S354/T355 phosphorylation is a key regulator of 5-HT4 desensitization, β-arrestin recruitment and internalization.

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Alternative Names 5-HT4, 5-Hydroxytryptamine Receptor 4, Serotonin Receptor... more

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Alternative Names	5-HT4, 5-Hydroxytryptamine Receptor 4, Serotonin Receptor 4
IUPHAR Target ID	9
UniProt ID	Q13639
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human 5-HT4 around the phosphorylation site of Ser354/Thr355.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine354/Threonine355 phosphorylation of the 5-Hydroxytryptamine... more

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Figure 1. Agonist-induced Serine354/Threonine355 phosphorylation of the 5-Hydroxytryptamine Receptor 4. Upper panel, HEK293 cells stably expressing the 5-Hydroxytryptamine Receptor 4 (5-HT4) were either not exposed or exposed to 100 µM serotonin or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS354/pT355-5-HT4 antibody (7TM0009C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-5-HT4 antibody to confirm equal loading of the gel.

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