pT404-β1 (phospho-β1-Adrenoceptor Antibody)

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Agonist-induced Threonine404 phosphorylation of the β1-Adrenoceptor

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Order number: 7TM0028A
Content: 100 µl
Host: Rabbit

Threonine404 (T404) is a major phosphorylation site of the β1 adrenoceptor. The pT404-β1... more

Threonine404 (T404) is a major phosphorylation site of the β1 adrenoceptor. The pT404-β1 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. T404 phosphorylation is primarily mediated by GRKs and is a key regulator of β1 desensitization, β-arrestin recruitment and internalization.

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Alternative Names β1, B1AR, ADRB1R, β1 Adrenergic... more

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Alternative Names	β1, B1AR, ADRB1R, β1 Adrenergic Receptor, β1-Adrenoceptor
IUPHAR Target ID	28
UniProt ID	P08588
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human β1 around the phosphorylation site of Thr404.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine404 phosphorylation of the β1-Adrenoceptor. Upper... more

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Figure 1. Agonist-induced Threonine404 phosphorylation of the β1-Adrenoceptor. Upper panel, HEK293 cells stably expressing the β1-Adrenoceptor (β1) were either not exposed or exposed to 10 µM of specific β1-adrenoceptor agonist Norepinephrene for 30 minutes. Cells were lysed and immunoblotted with the anti-pT404-β1 antibody (7TM0028A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-β1 antibody (7TM0028N) at a dilution of 1:1000 to confirm equal loading of the gel.

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