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pS331/pT332-AT1 (phospho-Angiotensin Receptor 1 Antibody)

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Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1
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  • 7TM0034A
  • 100 µl
  • Rabbit
Serine331/Threonine332 (S331/T332) is major phosphorylation site of the Angiotensin Receptor 1... more

Serine331/Threonine332 (S331/T332) is major phosphorylation site of the Angiotensin Receptor 1 (AT1). The pS331/pT332-AT1 antibody detects phosphorylation in response to agonists. S331/T332 phosphorylation is likely to be involved in efficient ligand sequestration by AT1.

  Alternative Names AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B... more

 

Alternative Names AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B Angiotensin II Receptor
IUPHAR Target ID  34
UniProt ID  P30556
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human AT1 around the phosphorylation site of Ser331/Thr332.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1.... more

Figure 1. Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Angiotensin receptor 1 (AT1) were either not exposed or exposed to 100 nM Angiotensin II for 30 minutes. Cells were lysed and immunoblotted with the anti-pS331/pT332-AT1 antibody (7TM0034A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-AT1 antibody to confirm equal loading of the gel.

 

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