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- Order number: 7TM0036B
- Content: 100 µl
- Host: Rabbit
Serine343/Serine345 (S343/S345) is major phosphorylation site of the Apelin Receptor (APLNR). The pS343/pS345-APLNR antibody detects phosphorylation in response to agonists. S343/S345 phosphorylation is likely to be involved in efficient ligand sequestration by APLNR.
Alternative Names | APLNR, Angiotensin Receptor-like 1 |
IUPHAR Target ID | 36 |
UniProt ID | P35414 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human APLNR around the phosphorylation site of Ser343/Ser345. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine343/Serine345 phosphorylation of the Apelin Receptor. Upper panel, HEK293 cells stably expressing the Apelin Receptor (APLNR) were either not exposed or exposed to 100 nM Apelin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS343/pS345-APLNR antibody (7TM0036B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-APLNR antibody to confirm equal loading of the gel.