APLNR (non-phospho), Apelin Receptor Antibody

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Preview: Validation of the APLN Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of APLN Receptor in human pancreas.

Validation of the APLN Receptor in transfected HEK293 cells

Immunohistochemical identification of APLN Receptor in human pancreas.

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Order number: 7TM0036N
Content: 100 µl
Host: Rabbit

The non-phospho-APLNR antibody is directed against the distal end of the carboxyl-terminal tail... more

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The non-phospho-APLNR antibody is directed against the distal end of the carboxyl-terminal tail of human apelin receptor. It also detects APLNR in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total APLNR in Western blots independent of phosphorylation. The APLNR antibody can also be used to isolate and enrich APLNR receptors from tissue lysates.

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Alternative Names APLNR, Angiotensin Receptor-like 1 IUPHAR Target... more

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Alternative Names	APLNR, Angiotensin Receptor-like 1
IUPHAR Target ID	36
Uniprot ID	P35414
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human APLNR.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the APLN Receptor in transfected HEK293 cells. Native HEK293 cells... more

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Figure 1. Validation of the APLN Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the APLN Receptor (APLNR) were lysed and immunoblotted with the phosphorylation-independent anti-APLNR antibody (7TM0036N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of APLN Receptor in human pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-APLNR (non-phospho-Apelin Receptor) antibody (7TM0036N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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