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B2 (non-phospho-Bradykinin Receptor 2 Antibody)

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  • 7TM0042N
  • 100 µl
  • Rabbit
The non-phospho-B2 receptor antibody is directed against the distal part of the... more

The non-phospho-B2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human B2. It can be used to detect total B2 receptors in Western blots independent of phosphorylation. The non-phospho-B2 antibody can also be used to isolate and enrich B2 receptors from cell and tissue lysates. It also detects B2 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names B2BKR, BK-2 Receptor, Bradykinin Receptor 2 IUPHAR... more

 

Alternative Names B2BKR, BK-2 Receptor, Bradykinin Receptor 2
IUPHAR Target ID  42
UniProt ID  P30411
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents the carboxyl-terminal tail of human B2.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Bradykinin  Receptor 2 in transfected HEK293 cells. Native... more

Figure 1. Validation of the Bradykinin  Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Bradykinin Receptor 2 (B2) were lysed and immunoblotted with the phosphorylation-independent anti-B2 antibody (7TM0042N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Bradykinin Receptor 2 in pancreatic islets. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated with anti-B2 (Bradykinin Receptor 2) antibody (7TM042N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, B2 receptors were detected in pancreatic islets.

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