B2 (non-phospho-Bradykinin Receptor 2 Antibody)

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Preview: Validation of the Bradykinin Receptor 2 in transfected HEK293 cells

Preview: Immunohistochemical identification of Bradykinin Receptor 2 in pancreatic islets

Validation of the Bradykinin Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of Bradykinin Receptor 2 in pancreatic islets

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Order number: 7TM0042N
Content: 100 µl
Host: Rabbit

The non-phospho-B2 receptor antibody is directed against the distal part of the... more

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The non-phospho-B2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human B2. It can be used to detect total B2 receptors in Western blots independent of phosphorylation. The non-phospho-B2 antibody can also be used to isolate and enrich B2 receptors from cell and tissue lysates. It also detects B2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names B2BKR, BK-2 Receptor, Bradykinin Receptor 2 IUPHAR... more

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Alternative Names	B2BKR, BK-2 Receptor, Bradykinin Receptor 2
IUPHAR Target ID	42
UniProt ID	P30411
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human B2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Bradykinin Receptor 2 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Bradykinin Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Bradykinin Receptor 2 (B2) were lysed and immunoblotted with the phosphorylation-independent anti-B2 antibody (7TM0042N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Bradykinin Receptor 2 in pancreatic islets. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated with anti-B2 (Bradykinin Receptor 2) antibody (7TM042N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, B2 receptors were detected in pancreatic islets.

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