pS425-CB1 (phospho-Cannabinoid Receptor 1 Antibody)

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Agonist-induced Serine425 phosphorylation of the Cannabinoid Receptor 1

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Order number: 7TM0056A
Content: 100 µl
Host: Rabbit

Serine425 (S425) is major phosphorylation site of the Cannabinoid Receptor 1 (CB1). The... more

Serine425 (S425) is major phosphorylation site of the Cannabinoid Receptor 1 (CB1). The pS425-CB1 antibody detects phosphorylation in response to agonists. S425 phosphorylation is likely to be involved in efficient ligand sequestration by CB1.

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Alternative Names CB-R, Central Cannabinoid Receptor IUPHAR Target... more

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Alternative Names	CB-R, Central Cannabinoid Receptor
IUPHAR Target ID	56
UniProt ID	P21554
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CB1 around the phosphorylation site of Ser425.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine425 phosphorylation of the Cannabinoid Receptor 1. Upper... more

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Figure 1. Agonist-induced Serine425 phosphorylation of the Cannabinoid Receptor 1. Upper panel, HEK293 cells stably expressing the CB1-Receptor (CB1) were either not exposed or exposed to 10 µM of CB1-Receptor agonists CP55940 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS425-CB1 antibody (7TM0056A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CB1 antibody to confirm equal loading of the gel.

Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and... more

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Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and cannabinoid drug effects using phosphosite-specific GPCR antibodies. Commun Biol. 2023 Apr 15;6(1):419. doi: 10.1038/s42003-023-04786-2. PMID: 37061609; PMCID: PMC10105690.

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