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pS357/pS358-CMKLR1 (phospho-Chemerin Receptor 1 Antibody)

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Agonist-induced Serine357/Serine358 phosphorylation of Chemerin Receptor 1.
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  • 7TM0079C
  • 100 µl
  • Rabbit
Serine357/Serine358 (S357/S358) is a major phosphorylation site of the Chemerin receptor 1... more

Serine357/Serine358 (S357/S358) is a major phosphorylation site of the Chemerin receptor 1 (CMKLR1). The pS357/pS358-CMKLR1 antibody detects phosphorylation in response to high-efficacy agonists. S357/S358 phosphorylation is a key regulator of CMKLR1 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CMKLR1, Chemerin 1 , Chemokine Receptor-like 1, Resolvin... more

 

Alternative Names CMKLR1, Chemerin1, Chemokine Receptor-like 1, Resolvin E1 Receptor, TIG2 Receptor
IUPHAR Target ID  79
UniProt ID  Q99788
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CMKLR1 around the phosphorylation site Ser357/Ser358.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine357/Serine358 phosphorylation of Chemerin Receptor 1. Upper... more

Figure 1. Agonist-induced Serine357/Serine358 phosphorylation of Chemerin Receptor 1. Upper panel, HEK293 cells stably expressing the Chemerin Receptor (CMKLR1) were either not exposed or exposed to 100 nM Chemerin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS357/pS358-CMKLR1 antibody (7TM0079C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CMKLR1 antibody to confirm equal loading of the gel.

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